ABSTRACT
Background: Helicobactor pylori [H. pylori] virulence markers would be useful to predict peptic ulcer disease [PUD] or gastric cancer
Aim: In Egypt, since inadequate data are present regarding H. pylori virulence-related genes in different age group patients with gastro-duodenal diseases, it becomes crucial to study the clinical status of cagA, vacA and iceA1 genotypes of H. pylori strains recovered from patients with dyspepsia
Subjects and methods: The study included 113 dyspeptic patients who were exposed to upper gastrointestinal endoscopic examination. Four antral biopsies were obtained from each patient for the analysis of H. pylori infection by rapid urease test and detection of 16S rRNA
Results: Sixty [53.1%] patients were confirmed to be infected with H. pylori. Upon endoscopy, gastritis was revealed in 27 patients [45%] and10 patients [16.7%] had PUD. Of the 60 H. pylori strains, 39 [65%] had at least one virulence gene. Six different genotypic forms were recognized; vacA [9/60], iceA1 [1/60], vacA/cagA [7/60], vacA/iceA1 [13/60], vacA/cagA/iceA1 [8/60] only one of cagA/iceA type and we could not detect cagA. The overall vacA, iceA1and cagA genes identified were 61.6%, 38.8%, 26.6% respectively, by PCR-based molecular testing. The vacA gene status was highly significant related to gastritis patient [P ? 0.036]. The vacA s1m1 and s2m2 alleles were significantly found in 50% of H. pylori infected patients with PUD and with gastritis 57.1% respectively [P
Conclusion: In conclusion, the main genotype combinations in the studied Egyptian patients were; vacAs2m2/iceA1, vacAs1m1/cagA, mostly associated with gastritis, and vacAs1/cagA/icA, mainly in PUD. The less virulent [s2, s2m2] H. pylori genotypes were found in patients aged over 43 years
ABSTRACT
Introduction: the present study was carried out to evaluate the effect of the non-steroidal anti-inflammatory drug diclofenac sodium [DS] on the fetuses of albino mice from the morphological and skeletal points of view
Material and methods: sixty adult pregnant female mice were used in the present study. They were allocated into 6 groups [10 mice each]. The first two groups served as control and were injected intraperitoneally [i.p.] with the solvent of the drug, and the 3rd and 5th groups were treated with 1.5 and 3mg/kg body weight of diclofenac sodium for 6 days [ gestation days 1-6 ], respectively ; the 4th and 6th groups were treated with 1.5and 3mg/kg body weight of the drug for 8 days [ gestation days 7-14], respectively
Results: the morphological examination of the fetuses of treated groups showed conspicuous decrease in the average body weight and body length in all treated groups. The fetuses maternally treated with the drug showed noticeable external morphological malformations and their skeletons exhibited mild retardation in skeletal elements
In conclusion: the non-steroidal anti-inflammatory drug diclofenac sodium had exerted marked morphological malformations and mild skeletal alterations in mice fetuses maternally treated during different periods of gestation
ABSTRACT
Stem cells are present in all self-renewing tissues and have unique properties. In certain pathological conditions, the limbal stem cells may be destroyed partially or completely resulting in varying degrees of stem cells deficiency with conjunctivalization of the cornea with vascularization. To evaluate the outcome of autologous limbal stem cell transplantation in ocular surface reconstruction. Forty two randomly chosen eyes with partial or complete unilateral limbal deficiency treated by autologous limbal stem cell transplantation. There were 18 males and 24 females with mean age 46.8 +/- 14.4 years. The indications for intervention included Bowen's disease, primary and recurrent pterygium, contact lens induced keratopathy, persistent epithelial defect and chemical injury. The mean preoperative best corrected visual acuity was 0.49 +/- 0.29. While the mean postoperative best corrected visual acuity was 0.74 +/- 0.21. There was a highly statistically significant difference between the results. Autologous limbal cell transplantation is an effective procedure in reconstructing of the ocular surface for ocular disorders associated with partial or diffuse limbal stem cell deficiency as it could be carried out from the same or contralateral healthy eye. It is a safe technique with minimal complications